Asymmetrische Fluß Feld-Fluß-Fraktionierung in Verbindung mit Mehrwinkel-Lichtstreudetektion Eine neue bedeutende Methode der pharmazeutischen Analytik Christian Augsten und Karsten Mäder Universität Halle-Wittenberg, Institut für Pharmazeutische Technologie und Biopharmazie, Halle/Saale Korrespondenz: Prof. Dr. rer. nat. habil. Karsten Mäder, Universität Halle-Wittenberg, Institut für Pharm. Technologie, Asymmetrical Flow Field-Flow Fractionation combined with Multi-Angle Light Scattering − a new Method of growing Importance in Pharmaceutical Analytics The general principles of Field-Flow Fractionation are known since several decades. Nevertheless, the sub-method Asymmetrical Flow Field-Flow Fractionation (aF-FFF or AF4) has gained increasing importance only very recently. Due to the combination with Multi-Angle Light Scattering (MALS) detectors the area of application was expanded especially in pharmaceutical fields. During the last years, aF-FFF/MALS has emerged as a very powerful and advantageous method to determine the molar mass of polymers and the size of nanoparticles. The equipment can be used for molar mass determinations instead of Gel Permeation Chromatography (GPC) or size determinations of particles in nanoscale range instead of or in addition to Dynamic Light Scattering (DLS). However, the interest in that technique strongly increased during the last years. It is the aim of this article to explain the principles of this method and to point out several main applications in pharmaceutics. Key words Feld-Fluß-Fraktionierung (FFF) • Mehrwinkel-Lichtstreudetektion (MALS) • Molekulargewicht • Molmasse • Nanopartikel • Partikelgröße |
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pharmind 2006, Nr. 12, Seite 1412
