Photoakustische Untersuchungen zur Wirkstoffpenetration aus Transdermalen Therapeutischen Systemen in HumanhautNutzung der Photoakustischen Spektroskopie zur Ermittlung von ortsaufgelösten Konzentrationsprofilen von Wirkstoffen in Humanhaut nach Applikation Transdermaler Therapeutischer Systeme Dieter Beckmann a, Gerald Lauckner a, Kai Schmidt a, Bodo Asmussen b, Michael Horstmann b, Andreas Koch b und Frank Theobald b Institut für Bioprozeß- und Analysenmeßtechnik e.V.a, Heiligenstadt, und LTS Lohmann Therapie-Systeme AG b, Andernach Photoacoustic Investigations on the Penetration of Drugs from Transdermal Therapeutic Systems through Human Skin / Use of photoacoustic spectroscopy to obtain concentration-depth-profiles of drugs in human skin after the application of transdermal therapeutic systems The determination of drug penetration through human skin in dependence of time is of major importance for the prediction of the systemic/local availability of drugs after transdermal application. In the presented investigations transdermal therapeutic systems (TTS) containing Estradiol (Climara) and 5-Fluoruracil (Actino-Hermal) were applied to human skin for 24 hours. After 24 hours the patches were removed and the drug content of both components was measured by photo-acoustic measure-ment. By photoacoustic measurements the drug concentration is measured by using pulsed laser-light which is emitted on human skin. A part of the energy which is absorbed by the drug molecules is emitted as heat and can be detected on skin surface as acoustic wave by a dedicated measurement-cell. It could be shown by the investigations that by use of photo-acoustic measurement concentration-depth-profiles of the drug within the human skin could be obtained. In advantage of the routinely used analytical methods the photo-acoustic measurement is distinguished by the fact that it is representing a non-invasive analytical tool, that does not require time consuming sample preparation Key words Estradiol · 5-Fluoruracil · Humanhaut · In-vitro-Penetration · Photoakustik · Transdermale Therapeutische Systeme |
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pharmind 2002, Nr. 3, Seite 271
